The isolation culture of tubercle bacilli from resected lung lesions

Streptomycin sensitivity of tubercle bacilli from resected lungs.

Selective media in the isolation of tubercle bacilli from tissues.

Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture me...

Brief communication: rapid culture of tubercle bacilli.

One of the biggest obstacles to the correct diagnosis and efficient treatment of tuberculosis is the absence of a rapid technique for culturing tubercle bacilli and for testing their susceptibility to antituberculosis drugs. Current procedures typically take 6-10 weeks to perform. This article describes a simple, rapid, reliable and cheap method of culturing tubercle bacilli using a liquid medi...

Langhans' giant cells and tubercle bacilli in 91 pulmonary tuberculous lesions resected after prolonged combined chemotherapy.

The Langhans’ giant cell described in 1868 is a large cell with numerous nuclei arranged in a ring around the periphery of an eosinophilic cytoplasm.1’ 2 The cytoplasm is homogeneous or granular and may contain inclusions such as Mycobacterium tuberculosis. The foreign body type giant cell can be distinguished by the presence of nuclei throughout the substance of the cytoplasm rather than at th...

The Proteins in Unheated Culture Filtrates of Human Tubercle Bacilli

1. Only two serologically different proteins were found in the unheated culture filtrates of both virulent and slightly virulent tubercle bacilli. One of them was the protein which had a sedimentation constant of 3.4 S, and the other was in filtrate fractions with a constant of 2 S. 2. That these proteins were distinct was demonstrated by three methods: quantitative precipitin and precipitin ab...